2VF8
Crystal structure of UvrA2 from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 149.632, 171.040, 204.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 102.060 - 3.000 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vf7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.964 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 50546 | |
| <I/σ(I)> | 11.2 | 2.1 |
| Completeness [%] | 96.4 | 98.2 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 17% PEG 3000, 0.1M CITRATE PH 5.2, 125MM AMMONIUM SULPHATE AND 1MM TCEP |
