2VEU
Crystal structure of protein tyrosine phosphatase 1B in complex with an isothiazolidinone-containing inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU MSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.280, 72.170, 88.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.400 |
| R-factor | 0.2327 |
| Rwork | 0.233 |
| R-free | 0.29230 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.654 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 7.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.070 |
| Number of reflections | 14220 |
| <I/σ(I)> | 12 |
| Completeness [%] | 87.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS PH 8.5, 17% PEG 20000, 100 MM MAGNESIUM |
