2VEQ
Insights into kinetochore-DNA interactions from the structure of Cep3p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.737, 83.737, 231.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.490 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.24600 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.250 |
| Number of reflections | 29594 | |
| <I/σ(I)> | 8.2 | 3 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100MM SODIUM CACODYLATE PH 6.5, 0.2M POTASSIUM THIOCYANATE, 12% PEG 4000 |
