2VEP
Crystal Structure Of The Full Length Bifunctional Enzyme Pria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.140, 65.140, 104.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.350 - 1.800 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.22400 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.480 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 23394 | |
| <I/σ(I)> | 19.3 | 2 |
| Completeness [%] | 95.7 | 94.3 |
| Redundancy | 7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 1.50 M AMMONIUM SULFATE AND 100 MM SODIUM CITRATE PH 4.8 |
