2VEE
Structure of protoglobin from Methanosarcina acetivorans C2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.930, 48.531, 161.072 |
| Unit cell angles | 90.00, 98.44, 90.00 |
Refinement procedure
| Resolution | 160.130 - 2.600 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2veb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.164 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.400 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.350 |
| Number of reflections | 47411 | |
| <I/σ(I)> | 9.6 | 2.7 |
| Completeness [%] | 97.3 | 98 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 15% ISOPROPANOL, 20% PEG 400, 0.1 M NA-HEPES PH 7.5 |
