2VEB
High resolution structure of protoglobin from Methanosarcina acetivorans C2A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-18 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.059, 49.271, 51.513 |
| Unit cell angles | 90.00, 92.88, 90.00 |
Refinement procedure
| Resolution | 51.430 - 1.300 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.19000 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.400 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 48188 | |
| <I/σ(I)> | 24.7 | 7.5 |
| Completeness [%] | 97.8 | 99.8 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 0.4 M MONOBASIC AMMONIUM PHOSPHATE, pH 4.2 |
