2VE8
Xray structure of FtsK gamma domain (P. aeruginosa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.494, 58.496, 95.408 |
| Unit cell angles | 90.00, 92.51, 90.00 |
Refinement procedure
| Resolution | 95.350 - 1.400 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j5o |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.332 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.090 | 0.530 |
| Number of reflections | 95599 | |
| <I/σ(I)> | 13.1 | 3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
