2VDI
Crystal structure of Chlamydomonas reinhardtii Rubisco with a large- subunit C192S mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.831, 178.206, 122.917 |
| Unit cell angles | 90.00, 117.76, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gk8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.114 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.120 | 0.330 |
| Number of reflections | 118474 | |
| <I/σ(I)> | 10.4 | 3.2 |
| Completeness [%] | 88.7 | 88.2 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
