2VC6
Structure of MosA from S. meliloti with pyruvate bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2006-10-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.900, 138.700, 123.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 1.950 |
| R-factor | 0.214 |
| Rwork | 0.280 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dhp |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.288 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.750 | 2.140 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.110 | 0.290 |
| Number of reflections | 77820 | |
| <I/σ(I)> | 8.4 | 3.9 |
| Completeness [%] | 93.4 | 74.4 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 2M AMMONIUM SULFATE, 100 MM TRIS BUFFER PH = 8.0, AND 2% V/V PEG400 |
