2VC0

Feast or famine regulatory protein (Rv3291c)from M. tuberculosis complexed with L-Leucine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	298
Spacegroup name	P 42 21 2
Unit cell lengths	100.875, 100.875, 99.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.250 - 2.500
R-factor	0.214
Rwork	0.212
R-free	0.25700
Structure solution method	OTHER
Starting model (for MR)	NONE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.095	0.520
Number of reflections	64899
<I/σ(I)>	11	2.3
Completeness [%]	96.0	98.3
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295