2VBM

Tailspike protein of bacteriophage Sf6 complexed with tetrasaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-07-08
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	95.358, 95.358, 182.765
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.670 - 2.000
R-factor	0.192
Rwork	0.190
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vbk
RMSD bond length	0.012
RMSD bond angle	1.362
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	39641
<I/σ(I)>	17.4	7
Completeness [%]	99.7	97.2
Redundancy	4.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	VAPOR DIFFUSION HANGING DROP, 20 DEGR. CELSIUS; DROP: 3 MICROL RESERVOIR AND 3 MICROL PROTEIN SOLUTION; RESERVOIR: 0.1M MES, PH6.5, 16% PEG8000, 20MM MNCL2; PROTEIN: 10MG/ML, 10MM NA-PHOSPHATE,PH7.0, OCTASACCHARIDE CONCENTRATION/PROTEIN CONCENTATION 10:1; CRYOCONDITION: 0.1M MES, PH6.5, 25% PEG8000, 20MM MNCL2, 15% ETHYLENGLYCOL