2VBK
NATIVE TAILSPIKE PROTEIN OF BACTERIOPHAGE SF6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-18 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 96.231, 96.231, 182.433 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.920 - 1.250 |
| R-factor | 0.12 |
| Rwork | 0.119 |
| R-free | 0.14200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vbe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.664 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 169746 | |
| <I/σ(I)> | 11.6 | 2 |
| Completeness [%] | 97.4 | 79.8 |
| Redundancy | 2.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | CRYSTALLIZATION CONDITIONS: VAPOR DIFFUSION HANGING DROP,20 DEGR. CELSIUS; DROP: 1 MICROL RESERVOIR AND 1 MICROL PROTEIN SOLUTION; RESERVOIR: 0.1M MES, PH6.0,18% PEG8000, 20MM MNCL2; PROTEIN: 10MG/ML, 10MM NA-PHOSPHATE,PH7.0, pH 6.5 |
