2VBE

Tailspike protein of bacteriophage Sf6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	110
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	H 3
Unit cell lengths	95.910, 95.910, 182.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.500 - 1.980
R-factor	0.119
Rwork	0.117
R-free	0.14900
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.433
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE (RESOLVE)
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	2.060
High resolution limit [Å]	1.980	1.980
Rmerge	0.050	0.080
Number of reflections	39829
<I/σ(I)>	11.1	8
Completeness [%]	95.6	65.4
Redundancy	2.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	VAPOR DIFFUSION HANGING DROP 20 DEG CELSIUS DROP: 3 UL RESERVOIR SNF 3 UL PROTEIN RESERVOIR: 0.1 M MES PH 6.5, 25% PEG 8000, 20 MM MNCL2 PROTEIN: 5MG/ML IN 10 MM SODIUM PHOSPHATE PH 7 CRYOCONDITION: 0.1 M MES PH 6.5, 25 % PEG 8000, 20 MM MNCL2