2VAY
Calmodulin complexed with CaV1.1 IQ peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.920, 34.670, 62.980 |
| Unit cell angles | 90.00, 113.69, 90.00 |
Refinement procedure
| Resolution | 19.770 - 1.940 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f3y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.800 | 1.860 |
| Rmerge | 0.030 | 0.140 |
| Number of reflections | 15915 | |
| <I/σ(I)> | 12.1 | 3.65 |
| Completeness [%] | 89.9 | 74.9 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | HANGING DROP VAPOR DIFFUSION 1.5 UL 10 MG/ML COMPLEX IN 20 MM MOPS PH7.4, 150 MM NACL, 4 MM CACL2 MIX WITH 4.5 UL WELL SOLUTION, 32% PEG 3350, 50 MM TRIS PH 8.3, 50 MM MGCL2, 5 DAYS AT 20-22 DEGREES C |
