2VAW
FtsZ Pseudomonas aeruginosa GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 100.658, 100.658, 66.319 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.2063 |
| Rwork | 0.206 |
| R-free | 0.31590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ofu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.357 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.140 | 0.160 |
| Number of reflections | 8626 | |
| <I/σ(I)> | 11.1 | 3.16 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 3.7 | 3.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 0.1M BIS-TRIS PH 5.8, 26% PEG2000MME |
