2VAK
Crystal structure of the avian reovirus inner capsid protein sigmaA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.210, 129.909, 144.037 |
| Unit cell angles | 93.81, 105.05, 98.16 |
Refinement procedure
| Resolution | 29.850 - 2.340 |
| R-factor | 0.211 |
| Rwork | 0.210 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ej6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.470 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.110 | 0.300 |
| Number of reflections | 282973 | |
| <I/σ(I)> | 7.7 | 2.2 |
| Completeness [%] | 94.3 | 74.8 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 278 | 100 MM SODIUM CITRATE, SODIUM PHOSPHATE OR MES NAOH BUFFER PH 5.5 TO 6.5, 0.1 TO 0.6 M AMMONIUM SULPHATE 10 MM TRIS HCL, 1 MM EDTA SITTING DROP VAPOUR DIFFUSION AT 278 K |
