2VAJ

Crystal structure of NCAM2 Ig1 (I4122 cell unit)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-5
Synchrotron site	MAX II
Beamline	I911-5
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-14
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	47.279, 47.279, 196.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.197 - 2.701
R-factor	0.2287
Rwork	0.227
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NCAM IG1 PDB ENTRY 1EPF CHAIN A
RMSD bond length	0.007
RMSD bond angle	1.059
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.040	0.100
Number of reflections	3356
<I/σ(I)>	35.9	7.1
Completeness [%]	99.2	99.2
Redundancy	10.1	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1