2VAE
Fast maturing red fluorescent protein, DsRed.T4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.347, 79.510, 83.014 |
| Unit cell angles | 90.07, 96.87, 97.01 |
Refinement procedure
| Resolution | 32.440 - 1.640 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zgo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.650 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.050 | 0.140 |
| Number of reflections | 210765 | |
| <I/σ(I)> | 19.1 | 5.6 |
| Completeness [%] | 95.9 | 87.9 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 100 MM HEPES, PH 7.5, 22% POLYACRYLIC ACID, 200 MM MGCL2, HANGING DROPS, RT |
