2VAD
Monomeric red fluorescent protein, DsRed.M1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.892, 62.151, 81.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.340 - 1.590 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zgo |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.693 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 27397 | |
| <I/σ(I)> | 43.7 | 6.7 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 100 MM HEPES, PH 7.6, 25% PEG3350, 200 MM MGCL2, 5 MM ZNSO4, RT, HANGING DROP |
