2VAD

Monomeric red fluorescent protein, DsRed.M1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	38.892, 62.151, 81.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.340 - 1.590
R-factor	0.177
Rwork	0.176
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zgo
RMSD bond length	0.014
RMSD bond angle	1.693
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.650
High resolution limit [Å]	1.590	1.590
Rmerge	0.060	0.400
Number of reflections	27397
<I/σ(I)>	43.7	6.7
Completeness [%]	99.7	99.6
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6		100 MM HEPES, PH 7.6, 25% PEG3350, 200 MM MGCL2, 5 MM ZNSO4, RT, HANGING DROP