2VAC

Structure of N-terminal Actin Depolymerizing Factor homology (ADF-H) domain of human twinfilin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-08-02
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	35.817, 48.560, 70.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.172
Rwork	0.170
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m4j
RMSD bond length	0.013
RMSD bond angle	1.290
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.3.0040)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.100	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.070	0.540
Number of reflections	13653
<I/σ(I)>	24.4	2.5
Completeness [%]	97.5	94.9
Redundancy	6.8	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		0.01M ZINC CHLORIDE,10% ETHYLENE GLYCOL,20% PEG6000, 0.1M TRIS PH8.0