2VA1
Crystal structure of UMP kinase from Ureaplasma parvum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.753, 96.552, 96.270 |
| Unit cell angles | 90.00, 105.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a1f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.038 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.420 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 0.120 |
| Number of reflections | 48558 | |
| <I/σ(I)> | 11.4 | 3.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM FLUORIDE, 20% PEG 3350 |
