2V9U

Rim domain of main porin from Mycobacteria smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	139.377, 105.652, 102.963
Unit cell angles	90.00, 109.26, 90.00

Refinement procedure

Resolution	65.790 - 2.590
R-factor	0.274
Rwork	0.273
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.576
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.790	2.690
High resolution limit [Å]	2.590	2.590
Rmerge	0.060	0.210
Number of reflections	36299
<I/σ(I)>	10.18	4.1
Completeness [%]	82.6	87.1
Redundancy	2.17	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1