2V9R
First and second Ig domains from human Robo1 (Form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.460, 108.100, 25.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.261 |
| Rwork | 0.258 |
| R-free | 0.33500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v9q |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.200 |
| Number of reflections | 54927 | |
| <I/σ(I)> | 15.6 | 7 |
| Completeness [%] | 97.7 | 94.1 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 12% PEG 6000, 0.1M LISO4, 0.1M NA CITRATE, pH 5.6 |
