2V9O
L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A87M- T109F-E192A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 |
| Unit cell lengths | 86.193, 86.193, 89.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.950 - 1.950 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojr |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.267 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 47805 | |
| <I/σ(I)> | 13.45 | 3.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.33 | 4.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 50% (V/V) ETHYLENE GLYCOL, 0.1 M HEPES (PH 7.5), 0.2 M LITHIUM SULFATE |
