2V9N
L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A88F- E192A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.379, 106.417, 106.564 |
| Unit cell angles | 90.00, 126.76, 90.00 |
Refinement procedure
| Resolution | 51.030 - 1.400 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 268317 | |
| <I/σ(I)> | 7.25 | 2.88 |
| Completeness [%] | 91.0 | 94.4 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.9 | 25% (V/V) 1,2-PROPANEDIOL, 10% (V/V) GLYCEROL, 5% (W/V) PEG 3000, PHOSPHATE-CITRATE BUFFER (0.1M, PH 4.2) |
