2V9G

L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT Q6Y- L84W-E192A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	87.902, 100.825, 270.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.960 - 2.700
R-factor	0.218
Rwork	0.218
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gt7
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.670	2.670
Rmerge	0.110	0.380
Number of reflections	32949
<I/σ(I)>	9.77	3.4
Completeness [%]	95.2	85.6
Redundancy	3.63	3.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.7		1 M NA/K-TARTRATE, 0.1 M HEPES (PH 7.7)