2V9B
X-ray structure of viscotoxin B2 from Viscum album
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.819, 40.392, 44.694 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.050 |
| R-free | 0.17030 |
| Structure solution method | DIRECT METHODS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SADABS |
| Phasing software | SHELXD |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.150 |
| High resolution limit [Å] | 1.150 | 1.050 |
| Rmerge | 0.060 | 0.220 |
| Number of reflections | 34317 | |
| <I/σ(I)> | 25.8 | 12.6 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 21.34 | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 1UL HANGING DROP, 30 MG/ML PROTEIN, 0.2M AMSO4, 0.08M CACODYLATE PH 6.5, 25% PEG20000 |
