2V91
STRUCTURE OF STRICTOSIDINE SYNTHASE IN COMPLEX WITH STRICTOSIDINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-28 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 150.177, 150.177, 121.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.010 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fp8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.841 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.050 |
| High resolution limit [Å] | 3.010 | 3.000 |
| Rmerge | 0.130 | 0.760 |
| Number of reflections | 20423 | |
| <I/σ(I)> | 21.2 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.8 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.8M POTASSIUM SODIUM TARTRATE TETRAHYDRATE, 100MM HEPES-NA, 1MM TRYPTAMINE, PH 7.5, |
