2V8V
Crystal structure of mutant R322A of beta-alanine synthase from Saccharomyces kluyveri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.478, 84.903, 104.848 |
| Unit cell angles | 67.45, 67.81, 62.75 |
Refinement procedure
| Resolution | 45.000 - 2.900 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r43 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.368 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.420 |
| Number of reflections | 44094 | |
| <I/σ(I)> | 7 | 2.2 |
| Completeness [%] | 95.2 | 96.6 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | TRISODIUM CITRATE PH 6.0, DIOXANE,ACETONE, DTT, NA-HEPES PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
