2V8Q

Crystal structure of the regulatory fragment of mammalian AMPK in complexes with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	48.522, 119.390, 129.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.213
Rwork	0.212
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.439
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100
Rmerge	0.060
Number of reflections	42012
<I/σ(I)>	18.7
Completeness [%]	99.0
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1