2V8A
The structure of Thermosynechococcus elongatus allophycocyanin at 3.5 Angstroems.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-08 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 102.177, 102.177, 193.984 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.150 - 3.500 |
| R-factor | 0.261 |
| Rwork | 0.260 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1all |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.399 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.150 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.220 | 0.370 |
| Number of reflections | 22898 | |
| <I/σ(I)> | 2.7 | 1.68 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.52 | 4.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | HANGING DROP VAPOUR DIFFUSION OF EQUAL VOLUMES OF 10MG/ML PROTEIN SOLUTION AND 1M AMMONIUM SULFATE ABOVE A RESERVOIR OF 1 M AMMONIUM SULFATE. |
