2V7Y
Crystal structure of the molecular chaperone DnaK from Geobacillus kaustophilus HTA426 in post-ATP hydrolysis state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.753, 71.455, 183.864 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.580 - 2.370 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hsc 1dkz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.400 |
| Data reduction software | Blu-Ice (CONTROL SOFTWARE) |
| Data scaling software | SCALEPACK (HKL-2000) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.580 | 2.450 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 25994 | |
| <I/σ(I)> | 14.9 | 2 |
| Completeness [%] | 94.8 | 93.9 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 200 MM AMMONIUM CITRATE, PH 7.5, 20% (V/V) ISOPROPANOL, 15% (W/V) PEG3350 |
