2V7S
Crystal structure of the putative lipoprotein LppA from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.000, 49.820, 42.210 |
| Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.960 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.496 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.070 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.110 | 0.320 |
| Number of reflections | 15229 | |
| <I/σ(I)> | 8.6 | 3.7 |
| Completeness [%] | 97.8 | 85.6 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 20% PEG-3350, BICINE 0.1 M, PH 9, 50 MM MGCL2 |
