2V7P

Crystal structure of lactate dehydrogenase from Thermus Thermophilus HB8 (Holo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	151.500, 142.850, 59.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.160 - 2.100
R-factor	0.192
Rwork	0.192
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v6m
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.100	0.340
Number of reflections	74676
<I/σ(I)>	10.6	3.78
Completeness [%]	97.7	96.7
Redundancy	4.86	3.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1M MES PH 6, 15% PEG 6K, 10MM NADH, 5MM OXAMATE