2V7N
Unusual twinning in crystals of the CitS binding antibody Fab fragment f3p4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.790, 185.920, 102.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.920 |
| R-factor | 0.1552 |
| R-free | 0.20170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.023 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (1.3.1) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.370 |
| Number of reflections | 151552 | |
| <I/σ(I)> | 10.2 | 3.5 |
| Completeness [%] | 97.4 | 83.3 |
| Redundancy | 4.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 23% PEG4000, 50 MM LI2SO4, 50 MM NA2SO4, 50 MM N-(2ACETAMIDO) IMINODIACETIC ACID (ADA), PH 6.5 |
