2V7K
PrnB D-tryptophan complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.265, 79.546, 92.309 |
| Unit cell angles | 90.00, 103.42, 90.00 |
Refinement procedure
| Resolution | 89.800 - 1.700 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v7j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.112 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.670 |
| Number of reflections | 48852 | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
