2V7H
Crystal structure of an immunogen specific anti-mannopyranoside monoclonal antibody Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.021, 79.737, 132.276 |
| Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
| Resolution | 17.460 - 2.800 |
| R-factor | 0.2424 |
| Rwork | 0.242 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fab |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.758 |
| Data reduction software | AUTOMAR |
| Data scaling software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 19865 | |
| <I/σ(I)> | 7.3 | 1.2 |
| Completeness [%] | 95.9 | 94.4 |
| Redundancy | 3.25 | 3.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 50MM TRISCL, PH 7.4 WITH 20% PEG 4000 |
