2V7D
14-3-3 protein zeta in complex with Thr758 phosphorylated integrin beta2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 94.920, 94.920, 233.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.600 - 2.500 |
| R-factor | 0.2277 |
| Rwork | 0.228 |
| R-free | 0.27330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.169 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.100 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.096 | 0.566 |
| Number of reflections | 40994 | |
| <I/σ(I)> | 16.86 | 4.91 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9 | 8.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 18-19% PEG3350, 10MM CACL2, 1MM NICL2, 100MM TRIS PH8.5 |
