2V77
Crystal Structure of Human Carboxypeptidase A1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-07 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 129.565, 129.565, 90.856 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.603 |
R-factor | 0.1782 |
Rwork | 0.178 |
R-free | 0.19180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cpa |
RMSD bond length | 0.007 |
RMSD bond angle | 1.383 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.680 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.050 | |
Number of reflections | 101271 | |
<I/σ(I)> | 33.5 | |
Completeness [%] | 99.0 | 83.1 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 22% (W/V) PAY 5100, HEPES 0.1 M, MAGNESIUM CHLORIDE 0.02 M (PH 7.5) |