2V76
Crystal structure of the human dok1 PTB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2006-09-02 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.880, 100.168, 67.101 |
| Unit cell angles | 90.00, 117.34, 90.00 |
Refinement procedure
| Resolution | 59.660 - 1.600 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5t |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.557 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4) |
| Phasing software | PHASER (CCP4) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.980 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 59364 | |
| <I/σ(I)> | 22.5 | 2.5 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 7.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2M AMONIUM SULFATE, 2% PEG 400, 0.1M NA HEPES PH 7.5 |
