2V73
The structure of the family 40 CBM from C. perfringens NanJ in complex with a sialic acid containing molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.769, 63.186, 70.931 |
| Unit cell angles | 90.00, 91.02, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.386 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 16878 | |
| <I/σ(I)> | 13.7 | 3.7 |
| Completeness [%] | 92.2 | 92 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
