2V72
The structure of the family 32 CBM from C. perfringens NanJ in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.265, 45.860, 69.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.258 |
| Rwork | 0.251 |
| R-free | 0.32500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.253 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.260 |
| Number of reflections | 6309 | |
| <I/σ(I)> | 9.2 | 4.1 |
| Completeness [%] | 93.6 | 98.6 |
| Redundancy | 4.34 | 4.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
