2V6S
Medium resolution crystal structure of pterin-4a-carbinolamine dehydratase from Toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-18 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 119.750, 119.750, 49.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.220 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dcp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.136 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.690 | |
| High resolution limit [Å] | 2.220 | |
| Rmerge | 0.050 | 0.290 |
| Number of reflections | 17850 | |
| <I/σ(I)> | 11.1 | 2.6 |
| Completeness [%] | 98.6 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 1.4M AMMONIUM SULFATE, 1M LITHIUM SULFATE, 2 MM 7,8-DIHYDROBIOPTERIN, pH 7.6 |
