2V66
Crystal Structure of the coiled-coil domain of Ndel1 (a.a. 58 to 169) C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.431, 73.221, 69.026 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.100 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.31000 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.922 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.310 |
| Number of reflections | 300363 | |
| <I/σ(I)> | 5 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.5 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 4% PEG 8000, 50 MM SODIUM PHOSPHATE PH 6.8, 100 MM NACL. PROTEIN CONCENTRATION 20 MG/ML. CRYOBUFFER = RESERVOIR PLUS 17% PEG 400. |
