2V65
Apo LDH from the psychrophile C. gunnari
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 113.790, 113.790, 109.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.790 - 2.350 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v6a |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.100 | 0.210 |
| Number of reflections | 28796 | |
| <I/σ(I)> | 9.51 | 5.08 |
| Completeness [%] | 95.9 | 97.1 |
| Redundancy | 2.97 | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 277 | 0.1M HEPES PH 7.8, 0.2M PROLINE, 14% PEG 3350, 4 DEG C |
