2V5Y
Crystal structure of the receptor protein tyrosine phosphatase mu ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.336, 69.325, 97.487 |
| Unit cell angles | 90.00, 113.52, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.246 |
| Rwork | 0.242 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.160 | 0.610 |
| Number of reflections | 19030 | |
| <I/σ(I)> | 10.3 | 2.5 |
| Completeness [%] | 98.6 | 89 |
| Redundancy | 5.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100MM BIS-TRIS-PROPANE, PH 6.5 22.5% (W/V) PEG-SMEAR, 200MM K-THIOCYANATE |
