2V5U
I92A FLAVODOXIN FROM ANABAENA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-30 |
| Detector | BRUKER |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.960, 37.491, 90.602 |
| Unit cell angles | 90.00, 123.65, 90.00 |
Refinement procedure
| Resolution | 26.130 - 1.990 |
| R-factor | 0.193 |
| Rwork | 0.189 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1obo |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.062 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.580 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 21357 | |
| <I/σ(I)> | 20.03 | 4.27 |
| Completeness [%] | 94.4 | 83.2 |
| Redundancy | 9.91 | 4.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30% PEG4K, 100 MM TRIS/HCL PH 8.5, 0.2 M MGCL2 |
