2V5M
Structural basis for Dscam isoform specificity
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-08 |
Detector | ADSC CCD |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 99.172, 99.172, 163.991 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.20500 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.014 |
RMSD bond angle | 2.656 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.080 | 0.510 |
Number of reflections | 271422 | |
<I/σ(I)> | 23.4 | 1.9 |
Completeness [%] | 97.8 | 91 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.5 M AMMONIUM SULPHATE, 0.1 M HEPES PH 7.5 |