2V5L
Structures of the Open and Closed State of Trypanosomal Triosephosphate Isomerase: as Observed in a New Crystal Form: Implications for the Reaction Mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.600, 48.200, 130.700 |
| Unit cell angles | 90.00, 100.60, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.158 |
| Rwork | 0.158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| Data reduction software | XDS |
| Data scaling software | GRONINGEN (BIOMOL PACKAGE) |
| Refinement software | TNT (NULL) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.430 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | |
| Number of reflections | 20429 | |
| Completeness [%] | 81.8 | 41.8 |
| Redundancy | 1.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 18% PEG6000, 200 MM TRIS PH 8.8, 1MM EDTA, 1MM DTT AND 1MM NAN3 |
