2V5I
Structure of the receptor-binding protein of bacteriophage Det7: a podoviral tailspike in a myovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-27 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.433, 100.433, 330.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.142 |
| Rwork | 0.141 |
| R-free | 0.17000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tsp |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.250 |
| Number of reflections | 80189 | |
| <I/σ(I)> | 13.1 | 3.8 |
| Completeness [%] | 94.6 | 85.9 |
| Redundancy | 5.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 10 TO 30 % (W/V) PEG4000, 0.1 M SODIUM CITRATE PH 4.5, 0 TO 15 % (V/V) GLYCEROL |
